Effects of Osmotic Potential of Culture Medium on Differentiation, Growth and Tropane Alkaloid Production in Adventitious Root Cultures of Atropa belladonna.
نویسندگان
چکیده
منابع مشابه
Enhancement of Tropane Alkaloid Production among Several Clones and Explants Types of Hairy Root of Atropa belladonna L.
Agrobacterium rhizogenes (pRi), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. In our study four bacterial strains AR15834, A4, 9435 and C318 and three explants types leaf, stems and roots, were examined. Hairy roots were induced from roots, stems and leaf explants. The highest transformation efficiency of 77% was achieved by usi...
متن کاملImprovement of tropane alkaloids production in hairy root cultures of Atropa belladonna by overexpressing pmt and h6h genes
Atropa belladonna L. is the commercial plant to produce tropane alkaloids including hyoscyamine and scopolamine, which are widely used as anticholinergic agents. In the present study, the genes encoding the key enzymes including putrescine N-methyltransferase (PMT) and hyoscyamine 6β-hydroxylase (H6H) were simultaneously overexpressed in transgenic hairy root cultures of A. belladonna. All of t...
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هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Overexpression of tropinone reductases alters alkaloid composition in Atropa belladonna root cultures.
The medicinally applied tropane alkaloids hyoscyamine and scopolamine are produced in Atropa belladonna L. and in a small number of other Solanaceae. Calystegines are nortropane alkaloids that derive from a branching point in the tropane alkaloid biosynthetic pathway. In A. belladonna root cultures, calystegine molar concentration is 2-fold higher than that of hyoscyamine and scopolamine. In th...
متن کاملenhancement of tropane alkaloid production among several clones and explants types of hairy root of atropa belladonna l.
agrobacterium rhizogenes (pri), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. in our study four bacterial strains ar15834, a4, 9435 and c318 and three explants types leaf, stems and roots, were examined. hairy roots were induced from roots, stems and leaf explants. the highest transformation efficiency of 77% was achieved by usi...
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ژورنال
عنوان ژورنال: Shokubutsu Kojo Gakkaishi
سال: 1997
ISSN: 1880-3555,0918-6638
DOI: 10.2525/jshita.9.208